Coding
Part:BBa_K3757005:Design
Designed by: Erik Zimmer Group: iGEM21_Tuebingen (2021-09-26)
c_KR-12_1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A His6-tag has been embedded into loop 5 and the AMP KR-12 in loop 1. Includes Esp3I (Type IIS restriction enzyme) recognition sites for fast cloning and expression as a cyclic peptide in Nicotiana benthamiana.
Source
MCoTI-II from Momordica cochinchinensis, amino acid sequence found in Cybase (http://www.cybase.org.au/index.php?page=card&table=protein&id=3), reverse transcibed into DNA sequence with codon usage optimized for Nicotiana bethaniana. KR-12 is a fragment of the cathelicidin LL-37 from Homo sapiens, amino acid sequence was found on DBAASP ID: 146 and was also reverse transcribed into DNA sequence.